REAGENTS

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• Cellmatrix Type I-A (Wako Chemicals, #637-00653) VIEW

• Rat Collagen I (LV) Lower Viscosity (Trevigen - Cultrex, #3443-100-01) VIEW

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PLEASE NOTE: because of the volume of requests for the stable expressing Rspo1-Fc cell line, distribution has now been turned over to Millipore, a Sigma-Aldrich Brand. For purchase, order online (Catalog #SCC111).

 

Requests for reagents or mice should be sent to Calvin at cjkuo@stanford.edu. We typically require a simple MTA.

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PROTOCOLS

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• Adenovirus production protocol DOWNLOAD PDF

• Culture of 293T cells stably expressing RSpo1-Fc DOWNLOAD PDF

• Production of R-Spondin 1-conditioned medium - a Millipore Protocol VIEW

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SOME REVIEW ARTICLES

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• Cancer organoids 2.0: modelling the complexity of the tumour immune microenvironment. Roel Polak , Elisa T. Zhang, Calvin J. Kuo. Nature Reviews Cancer. 2024 Jul 8. https://doi.org/10.1038/s41568-024-00706-6. LINK
   

• Regulation of the Blood-Brain Barrier in Health and Disease. Cara C. Rada, Kanako  Yuki, Jie Ding, Calvin J. Kuo. Cold Spring Harb Perspect Med. 2023 Mar 27:a041191. doi: 10.1101/cshperspect.a041191. PMID: 36987582. LINK

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• Immune organoids: from tumor modeling to precision oncology. Vinh Dao, Kanako Yuki, Yuan-Hung Lo, Michitaka Nakano, Calvin J. Kuo. Trends In Cancer. 2022. 8 (10): 870-880. doi: 10.1016/j.trecan.2022.06.001. LINK

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• An expanded universe of cancer targets. William C Hahn, Joel S Bader, Theodore P Braun, Andrea Califano, Paul A Clemons, Brian J Druker, Andrew J Ewald, Haian Fu, Subhashini Jagu, Christopher J Kemp, William Kim, Calvin J Kuo, Michael McManus, Gordon B Mills, Xiulei Mo, Nidhi Sahni, Stuart L Schreiber, Jessica A Talamas, Pablo Tamayo, Jeffrey W Tyner, Bridget K Wagner, William A Weiss, Daniela S Gerhard. Cancer Target Discovery and Development Network. Cell 2021 Mar 4;184(5):1142-1155. doi: 10.1016/j.cell.2021.02.020. PMID: 33667368 

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• Applications of organoids for cancer biology and precision medicine.  Yuan-Hung Lo, Kasper Karlsson, Calvin J. Kuo. Nature Cancer, Volume 1, 761–773 (2020) 

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• Organoid Models of Tumor Immunology. Yuki K, Cheng N, Nakano M, Kuo CJ. Trends Immunol. 2020 Aug;41(8):652-664. doi: 10.1016/j.it.2020.06.010. Epub 2020 Jul 9. PMID: 32654925 

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• Dart, A. Organoid 2.0. Nature Reviews Cancer 19, 126-127 (2019).

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• Santos AJM, Lo YH, Mah AT, Kuo CJ. The Intestinal Stem Cell Niche: Homeostasis and Adaptations. Trends Cell Biol. 2018 Dec;28(12):1062-1078. doi: 10.1016/j.tcb.2018.08.001. Epub 2018 Sep 5. Review. PMID: 30195922.

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• Bankaitis ED, Ha A, Kuo CJ, Magness ST. Reserve Stem Cells in Intestinal Homeostasis and Injury. Gastroenterology. 2018 Nov;155(5):1348-1361. doi: 10.1053/j.gastro.2018.08.016. Epub 2018 Aug 15. Review. PMID: 30118745.

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• Neal JT, Kuo CJ. Organoids as Models for Neoplastic Transformation. Annu Rev Pathol. (2016) Feb 22. PMID: 26907527.

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• Cantrell MA, Kuo CJ. Organoid modeling for cancer precision medicine. Genome Med. (2015) Mar 31;7(1):32. doi: 10.1186/s13073-015-0158-y. eCollection 2015. PMID: 25825593.

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• Vallon M, Chang J, Zhang H, Kuo CJ. Developmental and pathological angiogenesis in the central nervous system. Cell Mol Life Sci. (2014) Apr 24. [Epub ahead of print] PMID: 24760128.

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• Reyes TF, Kosinski C and Kuo CJ. MicroRNA-mediated Regulation of Angiogenesis. Current Angiogenesis, 2013, 2, 40-53 (2013).

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• Yeung, T. Chia, L, Kosinski C. and Kuo CJ. The intestinal stem cell niche. CMLS Reviews, Aug;68(15):2513-23. (2011).

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• Wei K, Kuhnert F, Kuo CJ.. Recombinant adenovirus as a methodology for exploration of physiologic functions of growth factor pathways. J Mol Med (Berl). (2008) Feb;86(2):161-9. Epub 2007 Sep 22. Review. PMID: 17891365